• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    A method is provided for the determination of microbe concentrations by means of a plating method, wherein a sample and a medium are plated in a dish, the medium is allowed to solidify and the solidified plate is incubated, wherein the colonies in the plate are counted. According to the method the microbes are inoculated to a medium solidifying in a dish and in such a manner that its layer thickness varies in a controlled manner. A dish is provided which is suitable for the determination of microbe concentrations by means of a plating method and the bottom part of which has such a shape that a solidified layer is formed in the dish after the plating of the sample and the medium, the thickness of the layer varying in a controlled manner. <IMAGE>
    公开号:SU1724014A3
    申请号:SU874202094
    申请日:1987-02-20
    公开日:1992-03-30
    发明作者:Тапио Юхани Нордлунд Вильо
    申请人:Валио Мейериен Кескусосуусмиике (Фирма)；
    IPC主号:
    专利说明:

    FIG. five

    WITH
    The invention relates to methods for the cultivation and enumeration of microorganisms in their study and analysis in separate plates.
    The purpose of the invention is to accelerate the counting of microorganisms and increase its accuracy.
    FIG. 1 shows a cup with a sloping bottom, a longitudinal section; in fig. 2 and 3 - the same, with a curved bottom; in fig. 4 - the same, with a flat bottom and vertical partitions, a longitudinal section; in fig. 5 and 6 - the same, with a stepped bottom; in fig. 7 - the same, with a curved sloping bottom; in fig. 8.9 and 10 are views A, B and C in FIG. 1.2 and 3, respectively (distribution of colonies of microorganisms in a nutrient medium).
    The cup for cultivating and counting microorganisms includes a housing 1 with a bottom 2, made sloping to the horizontal plane (Fig. 1.5 and 7) or curved (Fig. 2 and 3), or provided with vertical partitions 3, forming compartments 4 for the culture medium (figure 4). The height of the partitions decreases stepwise in the direction from one side of the body 1 of the cup to the other.
    Inclined bottom 2 cups can be formed from stepped sections 5 (figure 5). The curved bottom of the 2 cup can also be formed from the stepped sections 6 (FIG. 6). Inclined bottom 2 cups when it is tilted to the horizontal plane can also be made curved (Fig. 7).
    The cultivation and enumeration of microorganisms in the dish is carried out as follows.
    Before starting the cultivation and counting of microorganisms, their sample is mixed with a molten nutrient medium, such as agar, and the specified amount of this mixture is poured into the cup, the bottom 2 of which is made according to one of these options. After the medium has hardened, a cup or several cups are incubated until microorganisms form colonies.
    The same can be done in a cup with vertical partitions 3 and compartments 4. Upon filling the cup with a mixture of medium and sample and setting it on a horizontal surface, a solidified layer is formed in the cup, the thickness of which varies in a controlled manner. The cups can be, for example, round, like regular Petri dishes, or rectangular. The thickness of the medium layer is set at the desired level due to the shape of the bottom surface of a cup of a curved or mathematically controlled non-spherical shape, which can be convex or concave. The bottom 2 of the round cup can also be made convex or concave, for example, when using cups with
    concentrated annular horizontal flat surfaces with a circular flat surface in the middle. The bottom 2 of the rectangular cup can rise linearly along an arc or according to some mathematically controlled principle. It is also possible to provide the bottom 2 of the rectangular cup with rising flat rectangular steps 5 and 6. The cup geometry can also be composed of partitions 3 of different heights located at its bottom 2, the minimum thickness of the medium in the cups is 1 mm, and the maximum is 10 mm.
    Since the shape of the bottom 2
    cups or the presence of partitions in it allow for obtaining a different thickness of the medium in one dish at once, there is no need for successive dilutions of samples of microorganisms to count their colonies.
    The counting of microorganisms using this plate is carried out by selecting areas of the medium with microorganisms at the bottom 2 or compartment 4, in which the picture
    colony distribution and concentration is best for counting.
    Results are calculated separately for each site by dividing the number of counted colonies of each site by the number, volume of sample, determined for each site. The number of colonies counted for each plot is converted into microbial density in the original sample using the following relationship:
    Puyishy "rlvvs6ra
    Quantity of original sample
    In accordance with ERLM after incubation at different density of microorganisms, the development of colonies is different. The smallest density is formed in thinner layers of agar, and the highest in thicker layers of agar, and varies from the geometry of the medium to area x, which have intermediate layer thicknesses. When the sample and medium are thoroughly mixed before sowing, their quantities are known, and the geometry of the medium in the cup is geometrically controlled, the concentration of microbes in the sample can be easily determined. Any information that complies with accepted microbiological standards can be used in the determination, and only those colonies that are clearly distinguishable in
    plots with different thickness of the layer in the cup. The gradient of the layer thickness determines the density gradient of the colonies and is mathematically converted to the number of colonies. At the seeding stage, it is important that the cups are positioned strictly horizontally.
    The use of cups of this design eliminates the need for successive dilutions, because the density of colonies is sufficiently low, optimal for counting at least in some areas of the medium due to a change in the thickness of its layer, which saves counting time. A saving of culture medium is also achieved, proportional to the number of consecutive dilutions in known dishes. In addition, the number of cups themselves required for parallel determinations is reduced.
    The cup design eliminates the need for counting colonies throughout the cup.
    Thus, this cup design, along with the acceleration of microbial counting, provides an increase in counting accuracy.
    权利要求:
    Claims (4)
    [1]
    Claim 1. A cup for cultivating and counting microorganisms, comprising a housing with a bottom, characterized in that, in order to speed up the counting and increase its accuracy, the bottom of the cup is inclined to the horizontal plane or curved, or provided with vertical partitions that form compartments for culture medium, while the height of these barriers decreases in steps from one side of the cup to the other ...
    [2]
    2. A cup as claimed in claim 1, in which the inclined bottom of the cup is formed of stepped sections.
    [3]
    3. A cup according to claim 1, characterized in that the concave bottom of the cup is formed from
    stepped sections.
    [4]
    4. A cup according to claim 1, characterized in that the sloping bottom is curved.
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    Fi.d
    the form
    Type B
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    同族专利:
    公开号 | 公开日
    ES2003227A6|1988-10-16|
    US5134064A|1992-07-28|
    IT8719422D0|1987-02-19|
    DE3705229C2|1993-07-22|
    FI72741B|1987-03-31|
    IT1203494B|1989-02-15|
    BE1000345A4|1988-11-08|
    AU591236B2|1989-11-30|
    SE8700724D0|1987-02-20|
    NO870694L|1987-08-24|
    NO870694D0|1987-02-20|
    NO171282B|1992-11-09|
    BR8700812A|1987-12-15|
    FI72741C|1987-07-10|
    GB2187474B|1990-08-15|
    FR2594848B1|1989-11-03|
    SG12491G|1991-04-05|
    DK83187A|1987-08-22|
    GB8703721D0|1987-03-25|
    NO171282C|1993-02-17|
    SE8700724L|1987-08-22|
    AU6905287A|1987-08-27|
    NZ219308A|1988-09-29|
    DE3705229A1|1987-08-27|
    FI860767A0|1986-02-21|
    FR2594848A1|1987-08-28|
    GB2187474A|1987-09-09|
    PL156162B1|1992-02-28|
    NL8700429A|1987-09-16|
    CS275876B6|1992-03-18|
    DK83187D0|1987-02-18|
    CH670100A5|1989-05-12|
    CA1290667C|1991-10-15|
    SE467412B|1992-07-13|
    JPS62232396A|1987-10-12|
    HK17191A|1991-03-22|
    DD254652A5|1988-03-02|
    PL264224A1|1988-02-04|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    US3097070A|1958-11-06|1963-07-09|Falcon Plastic Products|Plastic ware for scientific use|
    US3729382A|1970-12-09|1973-04-24|Becton Dickinson Co|Microorganism sampling dish|
    US3892632A|1971-06-02|1975-07-01|Us Health|Method and apparatus for plating and counting aerobic bacteria|
    US3787290A|1972-04-10|1974-01-22|S Kaye|Method and means for assaying biological factors demonstrating quantal response|
    FR2204689B1|1972-10-26|1975-01-03|Launey Pierre|
    GB1437404A|1973-09-24|1976-05-26|Kaye S|Method and means for assaying biological factors demonstratingquantal response|
    DE2351617A1|1973-10-15|1975-04-24|Saul Kaye|Quantitative microorganism concn determining appts - contg a number of containers having different volumetric capacities and exposed simultaneously to sampling|
    SE403382B|1974-03-12|1978-08-14|Orion Yhtyme Oy Orion Diagnost|INVESTIGATE THE EFFECT OF A BIOLOGICAL ACTIVE SUBJECT ON THE GROWTH OF MICRO-ORGANISMS CULTIVATED ON A SOLID OR YELLOW-CULTURED MEDIUM|
    GB1478575A|1974-08-14|1977-07-06|Canadian Patents Dev|Apparatus for enumerating microorganisms|
    JPS5653000B2|1975-10-21|1981-12-16|
    US4204045A|1978-02-15|1980-05-20|Orion-Yhtyma Oy|Device for examining microorganisms|
    CA1154614A|1981-07-03|1983-10-04|Robert Beriault|Searching and counting device|
    JPH0558705B2|1984-10-09|1993-08-27|Kobayashi Pharma|US5417576A|1993-11-12|1995-05-23|Iowa Methodist Medical Center|Means and method for microbiological growth and in situ observation with microscopes|
    WO1996029428A1|1995-03-20|1996-09-26|Echa Microbiology Limited|Microbial monitoring|
    JP4303798B2|1997-09-11|2009-07-29|ソニー株式会社|Imaging device, editing device, and editing system|
    US7338773B2|2000-04-14|2008-03-04|Millipore Corporation|Multiplexed assays of cell migration|
    US20080187949A1|2001-10-26|2008-08-07|Millipore Corporation|Multiplexed assays of cell migration|
    US20080207465A1|2002-10-28|2008-08-28|Millipore Corporation|Assay systems with adjustable fluid communication|
    AU2002363076A1|2001-10-26|2003-05-06|Virtual Arrays, Inc.|Assay systems with adjustable fluid communication|
    US7381375B2|2001-10-26|2008-06-03|Millipore Corporation|Assay systems with adjustable fluid communication|
    US6602704B1|2002-06-24|2003-08-05|Biomerieux, Inc.|Sample contact plate with latchable cover|
    US8053230B2|2006-09-07|2011-11-08|Nalge Nunc International Corporation|Culture dish with lid|
    DE102010006473B4|2010-01-29|2011-09-22|GMBU Gesellschaft zur Förderung von Medizin-, Bio- und Umwelttechnologien e.V.|Microbiological culture plate with integrated smear system|
    DE202010001635U1|2010-01-29|2010-05-27|GMBU Gesellschaft zur Förderung von Medizin-, Bio- und Umwelttechnologien e.V.|Microbiological culture plate with guide bars in the lower edge of the lid and with an integrated smear system|
    DE202010001636U1|2010-01-29|2010-05-27|GMBU Gesellschaft zur Förderung von Medizin-, Bio- und Umwelttechnologien e.V.|Microbiological culture plate with guide pins in the lower edge of the lid and an integrated smear system|
    DE202011106557U1|2011-10-07|2012-01-30|GMBU Gesellschaft zur Förderung von Medizin-, Bio- und Umwelttechnologien e.V.|Microbiological culture plate with integrated smear system|
    FR3005316B1|2013-05-03|2015-04-17|Biomerieux Sa|DEVICE AND METHOD FOR DISTRIBUTING A SUSPENSION OF MICROORGANISMS|
    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    FI860767A|FI72741C|1986-02-21|1986-02-21|FOERFARANDE FOER BESTAEMNING MIKROBKONCENTRATIONER MEDELST EN SMAELTAGARMETOD SAMT DAERI ANVAENDA SKAOLAR.|
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